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Updated: Jun 28, 2026
Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Chromatographic analysis of phospholipid and cholesterol oxidation
U J Krull1, M Thompson, A Arya
1Chemical Sensors Group, Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada.
Abstract:
Capillary thin layer and gas chromatographic methods for analysis of the extent of oxidation in phosphatidyl choline/cholesterol samples are described. Examples of systems suitable for qualitative and quantitative analysis, based on use of unmodified samples or of their derivatives, are illustrated. A method for concurrent quantitative determination of phospholipid and sterol without preseparation is introduced and is based on extension of a previous lipid trans-methylation technique.
