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Related Concept Videos

Urine Studies I: Urinalysis01:29

Urine Studies I: Urinalysis

Urinalysis is a widely used diagnostic test that analyzes urine's physical, chemical, and microscopic characteristics. Healthcare providers use it to detect and monitor various health conditions, including renal disease, urinary tract infections (UTIs), diabetes, and metabolic or systemic disorders.Components of UrinalysisUrinalysis consists of three primary components: physical, chemical, and microscopic examination. Each provides unique insights into the urine sample and, by extension, the...
Serum Studies: Renal Function Tests01:24

Serum Studies: Renal Function Tests

Renal function tests are crucial for assessing kidney health, monitoring disease progression, and evaluating the kidneys' efficiency in waste elimination, fluid balance, and electrolyte regulation. These tests offer critical insights into kidney function, even though routine measurements may appear normal until there is a significant decline in the glomerular filtration rate or GFR. Typically, signs of kidney impairment only become evident when the GFR falls to about 50% of its normal level.

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Selenium in human urine determination, speciation and concentration levels.

H J Robberecht1, H A Deelstra

  • 1Department of Pharmaceutical Sciences, University of Antwerp (UIA), Universiteitsplein, 1, 2610 Wilrijk, Belgium.

Talanta
|July 1, 1984
PubMed
Summary

This review covers methods for measuring selenium in urine, addressing challenges in sample collection and preparation. It summarizes current selenium concentration levels and speciation in human urine.

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Toxicology

Background:

  • Selenium is an essential trace element crucial for human health.
  • Urine analysis is a common method for assessing selenium exposure and nutritional status.
  • Challenges exist in accurately measuring selenium due to its complex chemistry and potential for contamination.

Purpose of the Study:

  • To review and synthesize current procedures for selenium determination in human urine samples.
  • To identify and discuss fundamental issues related to sample collection and treatment for accurate selenium analysis.
  • To summarize established concentration levels and speciation patterns of selenium in urine.

Main Methods:

  • Literature review of analytical methodologies for selenium determination.
  • Discussion of pre-analytical factors including sample collection, storage, and handling.
  • Analysis of sample digestion and preparation techniques relevant to urine matrices.
  • Overview of common analytical techniques such as Atomic Absorption Spectroscopy (AAS) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS).

Main Results:

  • Various methods exist for selenium determination, each with specific advantages and limitations.
  • Sample contamination and analyte loss during collection and treatment are significant concerns.
  • Selenium concentration levels in urine vary widely based on geographical location, diet, and health status.
  • Understanding selenium speciation (different chemical forms) is critical for interpreting biological significance.

Conclusions:

  • Accurate selenium measurement in urine requires careful attention to sampling and sample-treatment protocols.
  • Standardized procedures are needed to ensure reliable and comparable results across different studies.
  • Further research into selenium speciation in urine can enhance our understanding of selenium's role in health and disease.