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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
07:26

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants

Published on: January 1, 2016

Immunoassays in aqueous two-phase systems.

T G Ling1, B Mattiasson

  • 1Pure & Applied Biochemistry, Chemical Centre, P.O. Box 740, S-220 07 Lund, Sweden.

Talanta
|October 1, 1984
PubMed
Summary
This summary is machine-generated.

Aqueous two-phase systems offer a novel method for separating bound and free components in binding assays. This technique is easily automated, making it ideal for immobilized ligand or adsorbent-based separations.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Separation Science

Background:

  • Binding assays are crucial for quantifying molecular interactions.
  • Traditional separation methods can be time-consuming and complex.
  • Need for efficient and automatable separation techniques in biological and chemical analyses.

Purpose of the Study:

  • To introduce aqueous two-phase systems (ATPS) as a novel separation method for binding assays.
  • To highlight the convenience and automation potential of ATPS in separating bound from free fractions.
  • To demonstrate the applicability of ATPS for immobilized ligand or adsorbent-based separations.

Main Methods:

  • Utilized aqueous two-phase systems for partitioning assay components.
  • Investigated the separation of bound and free fractions.
  • Explored automation possibilities for ATPS-based binding assays.

Main Results:

  • Aqueous two-phase systems effectively separate bound from free fractions.
  • The procedure is convenient and amenable to automation.
  • ATPS are suitable for separations involving immobilized ligands or adsorbents.

Conclusions:

  • Aqueous two-phase systems present a valuable and efficient tool for binding assay separations.
  • The automation potential of ATPS enhances throughput and simplifies complex assays.
  • This method offers a versatile alternative for various separation challenges in analytical and biochemical research.