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A new fluorogenic thiol-selective reagent: N-{p-[2-(6-dimethylamino)benzofuranyl]phenyl}maleimide
K Nakashima1, H Akimoto, K Nishida
1Faculty of Pharmaceutical Sciences, Nagasaki University, Bunkyo-machi, Nagasaki 852, Japan.
Abstract:
N-{p-[2-(6-Dimethylamino)benzofuranyl]phenyl}maleimide (DBPM), has been synthesized and found to give fluorescent products when reacted with certain thiols. The reaction is very sensitive, and concentrations as low as 10(-9)M can be detected.
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