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Published on: January 6, 2016
Fluorimetric determination of boron at microgram level
1Analytical Chemistry Department, Faculty of Sciences, University of Zaragoza, Zaragoza, Spain.
A new reagent, 2-(2-hydroxybenzylidenimine)benzene-arsonic acid (HBBA), enables sensitive fluorimetric determination of boron. This method accurately quantifies boron in plant materials with a low detection limit.
Area of Science:
- Analytical Chemistry
- Environmental Science
Background:
- Accurate boron determination is crucial for environmental and agricultural monitoring.
- Existing fluorimetric methods for boron may lack sensitivity or require complex procedures.
Purpose of the Study:
- To develop and validate a novel reagent for sensitive boron quantification.
- To establish a reliable fluorimetric method for boron analysis in complex matrices like vegetal material.
Main Methods:
- Synthesis and characterization of 2-(2-hydroxybenzylidenimine)benzene-arsonic acid (HBBA).
- Fluorimetric analysis of boric acid using HBBA in 85% sulfuric acid at 90°C for 45 minutes.
- Method validation including calibration range and detection limit determination.
Main Results:
- HBBA is synthesized and characterized as a selective reagent for boron.
- A highly fluorescent compound is formed upon reaction of HBBA with boric acid under specific acidic and thermal conditions.
- The method demonstrates a linear calibration range of 0.1-8 µg/mL and a detection limit of 0.01 µg/mL.
Conclusions:
- HBBA is an effective reagent for the fluorimetric determination of boron.
- The developed method is sensitive and suitable for analyzing boron in vegetal samples.
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