Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Persistent Low-Output Neonatal Chylothorax: How Long Are We Justified to Wait for Pleurodesis in Partial Responders?

Pediatric pulmonology·2025
Same author

Crohn’s disease and extra intestinal granulomatous lesions.

Journal of biological regulators and homeostatic agents·2018
Same author

Colorectal cancer and inflammatory bowel diseases: effects of diet and antioxidants.

Journal of biological regulators and homeostatic agents·2017
Same author

Colorectal cancer: an update on the effects of lycopene on tumor progression and cell proliferation.

Journal of biological regulators and homeostatic agents·2017
Same author

Helicobacter pylori and Barrett’s esophagus: a protective factor or a real cause?

Journal of biological regulators and homeostatic agents·2017
Same author

The fingerprint of the human gastrointestinal tract microbiota: a hypothesis of molecular mapping.

Journal of biological regulators and homeostatic agents·2017

Related Experiment Video

Updated: Jun 28, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
08:25

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Fluorometric determination of nitrite.

P Damiani1, G Burini

  • 1Istituto di Chimica Bromatologica, Facoltà di Farmacia, Università degli Studi, 06100 Perugia, Italy.

Talanta
|August 1, 1986
PubMed
Summary
This summary is machine-generated.

A new fluorometric method accurately determines nitrite using 2,3-diaminonaphthalene. This sensitive technique offers a detection limit of 0.5 ng/ml, suitable for analyzing milk samples.

More Related Videos

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

Related Experiment Videos

Last Updated: Jun 28, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
08:25

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Food Science

Background:

  • Nitrite is a common analyte in environmental and food samples.
  • Accurate determination of nitrite is crucial for quality control and safety.
  • Existing methods for nitrite determination may lack sensitivity or simplicity.

Purpose of the Study:

  • To develop a sensitive, simple, and rapid fluorometric method for nitrite determination.
  • To establish the detection limit and applicability of the new method.
  • To validate the method using milk sample analysis.

Main Methods:

  • Fluorometric analysis based on the reaction of nitrite with 2,3-diaminonaphthalene.
  • Formation of a highly fluorescent 1-[H]-naphthotriazole compound in an alkaline medium.
  • Determination of the detection limit and testing on spiked milk samples.

Main Results:

  • The method provides a sensitive and rapid determination of nitrite.
  • The detection limit was found to be approximately 0.5 ng/ml.
  • The procedure was successfully applied to the analysis of nitrite in milk samples.

Conclusions:

  • The described fluorometric procedure is a reliable method for nitrite determination.
  • The method's sensitivity and simplicity make it suitable for routine analysis.
  • This technique offers a valuable tool for monitoring nitrite levels in various matrices.