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Published on: December 9, 2022
Analysis of anthranilic acid by liquid chromatography
T M Schmitt1, R J Ziegler, E S Muzher
1Central Research and Development, BASF Wyandotte Corporation, Wyandotte, MI 48192, USA.
Abstract:
Analytical methods for the assay of anthranilic acid and for determination of the impurities methyl anthranilate, anthranoylanthranilic acid and 3- and 4-aminobenzoic acid are described. A Microbondapak C18 column is used for both the assay and the impurity determination. The assay is based on isocratic development with a mobile phase of 35:65 v v methanol/pH-3 phosphate buffer, with benzoic acid as internal standard. The impurities are separated by gradient elution. The standard deviation of the assay method is about 1% and the limit of detection for the impurities is about 0.01%.
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