Trace zinc determination by synchronous derivative fluorimetry.

Area of Science:

• Analytical Chemistry
• Environmental Science
• Biochemistry

Background:

• Accurate quantification of trace zinc is crucial for biological and environmental monitoring.
• Existing fluorimetric methods may lack sufficient sensitivity or selectivity for complex matrices.

Purpose of the Study:

• To develop and validate a sensitive and selective synchronous-scanning derivative spectrofluorimetric method for zinc determination.
• To compare the analytical performance of the new method with the conventional fluorimetric approach.
• To apply the developed methods for trace zinc analysis in real-world samples.

Main Methods:

• Synchronous-scanning derivative spectrofluorimetry utilizing 2-furaldehyde 2-pyridylhydrazone (FAPH) as a chelating agent.
• Formation of a fluorescent zinc-FAPH chelate.
• Analysis of pig liver tissue and environmental fume samples.

Main Results:

• The developed method demonstrated high sensitivity and selectivity for zinc determination.
• Detection limits were in the nanogram per milliliter range.
• The coefficient of variation was consistently below 2% for both methods.
• Successful application to trace zinc determination in pig liver and environmental fume samples.

Conclusions:

• Synchronous-scanning derivative spectrofluorimetry with FAPH provides a robust and efficient method for trace zinc analysis.
• The method is suitable for analyzing zinc in complex biological and environmental matrices.
• This technique offers an advantageous alternative to conventional fluorimetric methods for zinc quantification.