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Updated: Jun 28, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

[Not Available].

M Sarbar¹, S M Golabi, M H Pournaghi-Azar

¹Laboratoire de chimie analytique, Faculté des Sciences, Université de Tabriz, Tabriz, Iran.

|November 1, 1986

Summary

This summary is machine-generated.

This study investigated two organic compounds, DFPO and DTPO, as acid-base indicators in anhydrous acetic acid. Spectrophotometry determined their pK(i) values, assessing their effectiveness in acid-base titrations.

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Area of Science:

Organic Chemistry
Analytical Chemistry
Spectroscopy

Context:

Acid-base indicators are crucial for determining titration endpoints.
Organic compounds with conjugated systems often exhibit indicator properties.
Anhydrous acetic acid provides a non-aqueous medium for studying indicator behavior.

Purpose:

To investigate the acid-base indicator properties of 1,5-di[2-furyl]-1,4-pentadien-3-one (DFPO) and 1,5-di[2-thienyl]-1,4-pentadien-3-one (DTPO).
To determine the pK(i) values of DFPO and DTPO in anhydrous acetic acid using spectrophotometry.
To evaluate the efficacy of DFPO and DTPO in visual and colorimetric detection of acid-base titration endpoints.

Summary:

The acid-base indicator behavior of DFPO and DTPO was studied in anhydrous acetic acid with varying perchloric acid concentrations.

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Last Updated: Jun 28, 2026

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

Published on: November 15, 2013

Absorbance versus wavelength (A = f(lambda)) curves were plotted to analyze spectral changes.

Spectrophotometric determination of pK(i)(HClO(4))) values was performed, and the indicators' efficiency in titration endpoint detection was assessed.

Impact:

Provides quantitative data on the acid-base indicator properties of DFPO and DTPO.
Contributes to the understanding of non-aqueous acid-base titrations.
Offers potential new indicators for specific analytical applications in non-aqueous media.