Related Experiment Video
Updated: Jun 28, 2026

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
Published on: January 17, 2020
The effect of temperature on single-column ion-chromatography of metal ions
1Ames Laboratory and Department of Chemistry, Iowa State University, Ames, Iowa 50011, U.S.A.
Abstract:
Temperature is shown to be a valuable parameter for optimizing single-column ion-chromatographic separations of metal ions. With a perchloric acid eluent, retention times of bivalent metal ions decrease with an increase in system temperature, but with doubly protonated amines as eluents the retention times increase with an increase in temperature. Consequently, increasing the system temperature improves separations of bivalent metal ions when p-phenylenediamine dilhydrochloride is used as the eluent. Both conductivity detection and post-column reaction followed by spectrophotometric detection are suitable detection methods at above-ambient temperatures.
More Related Videos
Related Concept Videos
Ion-Exchange Chromatography
Principles Of Column Chromatography
Atomic Spectroscopy: Effects of Temperature
At thermal equilibrium, the relative populations of excited and ground state atoms can be estimated using the Maxwell–Boltzmann distribution. For example, an increase in temperature from...
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
Column Efficiency: Rate Theory
During elution, a solute molecule experiences numerous transitions between stationary and mobile phases, exhibiting irregular residence times in...
Gas Chromatography: Types of Detectors-I
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...

