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Updated: Jun 28, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
A new chelating ion-exchanger containing p-bromophenylhydroxamic acid as functional group-IV: column separations on a
Abstract:
A new chelating resin based on macroreticular acrylonitrile-divinylbenzene copolymer and containing hydroxamic acid functional groups has been synthesized. It is highly stable in acidic and alkaline solutions. The sorption characteristics of Cu(II), Cd(II), Pb(II), Zn(II), U(VI), Cr(VI), V(V), Co(II), Ni(II), Ca(II) and Mg(II) have been investigated over the pH range 1.0-6.0. The effect of various electrolytes at different ionic strengths on the K(d) values for Cu(II), Cd(II), Pb(II) and Zn(II) has been studied systematically. Chromatographic separations of copper(II) and nickel(II) from cobalt (II), and of uranium(VI) from chromium(VI) by selective sorption at controlled pH, have been developed. The ion-exchanger can be used for purification of inorganic salts, and analysis of brass and bauxite.
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