Related Experiment Video
Updated: Jun 28, 2026

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
New liquid-membrane electrodes for direct determination of atropine in pharmaceutical preparations
S S Hassan1, M A Ahmed, F S Tadros
1Department of Chemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
Abstract:
Solutions of atropinium 5-nitrobarbiturate in n-octanol and atropinium picrolonate in p-nitrotoluene are used as novel liquid ion-exchangers in electrodes that respond to the atropine cation. The performance characteristics of the two electrodes are almost identical: the response is linear for 10(-5)-10(-2)M atropine with a slope of 56.5 +/- 1 mV/concentration decade. The static response times are 30-90 sec and the potential readings are stable over the pH range 3-8. There is negligible interference from a number of inorganic and organic cations, and some common excipients. In the direct determination of 1-200 mug/ml of atropine, the average recovery with both electrodes is 98% (mean standard deviation 1.7%). Atropine has been determined in some pharmaceutical preparations with an average recovery of 98% (standard deviation 1.9%), the results being in agreement with those obtained by standard methods.
More Related Videos
08:56Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
Published on: November 22, 2024
09:10An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants
Published on: June 12, 2026
Related Concept Videos
Potentiometry: Membrane Electrodes
Direct-Acting Cholinergic Agonists: Pharmacokinetics
High-Performance Liquid Chromatography: Types of Detectors
Indirect-Acting Cholinergic Agonists: Pharmacokinetics
Reversible agents containing quaternary amines, such as neostigmine and edrophonium, are not easily absorbed orally because they are...
Direct-Acting Cholinergic Agonists: Therapeutic Uses