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Updated: Jun 28, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Computer-assisted optimization of HPLC with post-column reaction for the determination of amino-acids
S Al-Najafi1, C A Wellington, A P Wade
1The Chemistry Department, University College Swansea, Singleton Park, Swansea, Wales U.K.
Abstract:
Computer-assisted optimization of a high-performance liquid chromatograph and associated post-column reactor is reported for the determination of six amino-acids. First six, then seven, experimental variables were considered. Non-standard experimental conditions were found which gave significantly improved colour development in the ninhydrin reaction. The composite modified simplex method for experimental optimization and a novel system-response function facilitated rapid and simultaneous improvement of separation, sensitivity and analysis time. The approach described is directly applicable to many similar systems.
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