Related Experiment Video
Updated: Jun 28, 2026

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
Published on: March 4, 2017
Study of the physicochemical properties of aqueous dantrolene solutions by differential pulse polarography
M H Livertoux1, Z Jayyosi, A M Batt
1Université de Nancy I, Centre du Médicament, U.A. CNRS No. 597, 30 rue Lionnois, F-54000 Nancy, France.
Abstract:
Dantrolene is a skeletal-muscle relaxant which exists in three different forms over the pH range 0-14, corresponding to the cation H(2)A(+), neutral species HA and anion A(-). Dantrolene is slightly soluble in water and is polarographically reducible over the whole pH range. The acid dissociation constants of the ionic species were investigated by means of differential pulse polarography (H(2)A(+)/HA equilibrium with pK(a1) = 2.02 +/- 0.05) and potentiometry (HA/pK(a2) = 7.49 +/- 0.05). It was shown that differential pulse polarography is a valid alternative method when the pK(a) values of an electroactive drug cannot be determined by potentiometric or spectrophotometric methods. Polarography was also used to monitor the stability of the drug in acidic media and its extraction by chloroform.
Related Concept Videos
Voltammetric Techniques: Linear-Scan (E vs Time)
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Measuring Reaction Rates
Voltammetric Techniques: Pulse Voltammetry
Extraction: Partition and Distribution Coefficients
For extracting a solute from an aqueous phase into an organic...

