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Updated: Jun 28, 2026
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Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
Published on: August 22, 2017
Fluorometric determination of reserpine in dosage forms
1Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura 35516, Egypt.
Talanta
|September 1, 1988
Summary
A new fluorometric method accurately measures reserpine in bulk and dosage forms. This rapid technique uses hexa-amminecobalt(III) tricarbonatocobaltate oxidation for sensitive reserpine detection.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
Background:
- Reserpine analysis requires sensitive and rapid methods for quality control.
- Existing methods may lack the speed or sensitivity needed for routine pharmaceutical analysis.
Purpose of the Study:
- To develop a rapid and highly sensitive fluorometric procedure for reserpine determination.
- To validate the method for both bulk reserpine and its presence in various dosage forms.
Main Methods:
- Fluorometric analysis based on reserpine oxidation.
- Utilized hexa-amminecobalt(III) tricarbonatocobaltate in aqueous acetic acid.
- Measured greenish yellow fluorescence emission maximum at ~420 nm.
Main Results:
- Achieved high sensitivity with a linear dynamic range of 0.01-0.24 µg/ml.
- Demonstrated applicability to different pharmaceutical formulations.
- Method is rapid and suitable for routine determination.
Conclusions:
- The developed fluorometric method offers a sensitive and rapid approach for reserpine quantification.
- The procedure is effective for analyzing reserpine in bulk and pharmaceutical products.
- This method presents a viable alternative for quality control in pharmaceutical settings.
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