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Updated: Jun 28, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
Surface-active substrates for Raman and luminescence analysis
T Vo-Dinh1, G H Miller, J Bello
1Advanced Monitoring Development Group, Health and Safety Research Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6101, U.S.A.
Abstract:
This paper describes the development of active materials for optically enhanced Raman and fluorescence spectroscopy. The substrates for surface-enhanced Raman scattering investigated in this study involved silver-coated microspheres on glass plates. The effect of various experimental parameters, such as angle of incidence and excitation wavelength, were investigated. The substrate used for surface luminescence analysis consisted of a cellulose membrane coated with fumed silica microparticles, to enhance the sensitivity of analysis. Examples of analysis of benzo[a]pyrene and its derivatives are used to illustrate the efficacy of the analytical techniques.
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