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Updated: Jun 28, 2026

Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity
Published on: August 19, 2013
Comparison of algorithms for error-compensated kinetic determinations without prior knowledge of reaction orders or
1Department of Chemistry, Purdue University, West Lafayette, IN 47907, U.S.A.
Abstract:
This paper describes the evaluation of algorithms written for error-compensated kinetic determinations, that do not need prior knowledge of reaction orders or rate constants. Results are reported for the quantification of acetoacetate in aqueous solution and urine. In addition to examination by nonlinear and linearized versions of the proposed new algorithms, the kinetic data were processed by a first-order model for comparison purposes. All the models yielded linear relationships between computed absorbance change and concentration; the new algorithms yield virtually identical results that represent better fits to the kinetic data than those obtained with the first-order model. The ability of the new algorithms to detect errors in the models is briefly discussed.
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