Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Amperometry: Overview01:10

Amperometry: Overview

Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
Electrochemical Systems01:24

Electrochemical Systems

Electrochemical systems provide a fascinating insight into the dynamic interplay of charged species within various phases. One notable example is the interaction between a membrane permeable to K⁺ ions but not to Cl⁻ ions, separating an aqueous KCl solution from pure water. As K⁺ ions diffuse through the membrane, they generate net charges on each phase, leading to a potential difference between them.Similarly, when a piece of Zn is immersed in an aqueous ZnSO₄ solution, the Zn metal, composed...
Potentiometry: Types of Electrodes01:19

Potentiometry: Types of Electrodes

Reference electrodes serve as a stable reference point for potentiometric measurements, while indicator and working electrodes react to variations in the composition of a solution.
The Standard Hydrogen Electrode (SHE) is a widely used reference electrode that maintains zero potential across all temperatures. However, its need for a continuous hydrogen gas supply renders it impractical for everyday use.
An alternative to SHE is the Saturated Calomel Electrode (SCE). This electrode features an...
Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
Interfacial Electrochemical Methods: Overview01:06

Interfacial Electrochemical Methods: Overview

Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current passing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic changes in magnetic resonance imaging (MRI) features improve sensitivity for diagnosing small recurrent hepatocellular carcinoma after resection.

Clinical radiology·2026
Same author

[Clinical application effects of the "three-source and four-line method" in the design and harvest of anterolateral lower leg perforator flaps].

Zhonghua shao shang yu chuang mian xiu fu za zhi·2026
Same author

[Clinical application effects of indocyanine green angiography combined with color Doppler ultrasound in perforator localization of anterolateral thigh perforator flaps].

Zhonghua shao shang yu chuang mian xiu fu za zhi·2025
Same author

Multiple independent acquisitions of a metallophore-synthesis gene by plants through horizontal microbial gene transfer.

Nature communications·2025
Same author

[Interaction between gender and visceral adiposity index-associated risk of type 2 diabetes].

Zhonghua nei ke za zhi·2025
Same author

[Influencing factors and predictive model development for contralateral central lymph node metastasis in unilateral papillary thyroid microcarcinoma].

Zhonghua yi xue za zhi·2025
Same journal

Detection and cross-organ characterization of physiological response to microplastic stress in Panax ginseng based on hyperspectral imaging assisted with machine learning.

Talanta·2026
Same journal

Electron-driven proton transfer behaviors in oxidative DNA lesion 8-oxoguanine: A computational study.

Talanta·2026
Same journal

TiO<sub>2</sub> nanoengineering for high-performance photoelectrochemical biosensors: From fundamental strategies to frontier applications.

Talanta·2026
Same journal

CRISPR-based diagnostics for ESKAPE drug-resistant bacteria: From proof-of-concept to point-of-care.

Talanta·2026
Same journal

Integrated 3D printed wearable microfluidics with removable electrochemical sensor for morphine monitoring in sweat.

Talanta·2026
Same journal

Eu-doped Tourmaline@MOF-ZnO core-shell nanocomposites: Enabling UV-activated room-temperature and ppb-level n-butanol sensing.

Talanta·2026
See all related articles

Related Experiment Video

Updated: Jun 28, 2026

Dynamic Electrochemical Measurement of Chloride Ions
07:32

Dynamic Electrochemical Measurement of Chloride Ions

Published on: February 5, 2016

An electrochemical microsensor for chloride.

S Dong1, G Che

  • 1Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, People's Republic of China.

Talanta
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

A novel chloride-doped polypyrrole (PPY) microdisk electrode on carbon fibre (CF) offers sensitive detection. This optimized electrode provides a Nernstian response for chloride detection in serum samples.

More Related Videos

Fabrication of Thin Film Silver/Silver Chloride Electrodes with Finely Controlled Single Layer Silver Chloride
07:23

Fabrication of Thin Film Silver/Silver Chloride Electrodes with Finely Controlled Single Layer Silver Chloride

Published on: July 1, 2020

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
08:19

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Published on: June 1, 2012

Related Experiment Videos

Last Updated: Jun 28, 2026

Dynamic Electrochemical Measurement of Chloride Ions
07:32

Dynamic Electrochemical Measurement of Chloride Ions

Published on: February 5, 2016

Fabrication of Thin Film Silver/Silver Chloride Electrodes with Finely Controlled Single Layer Silver Chloride
07:23

Fabrication of Thin Film Silver/Silver Chloride Electrodes with Finely Controlled Single Layer Silver Chloride

Published on: July 1, 2020

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
08:19

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Published on: June 1, 2012

Area of Science:

  • Electrochemistry
  • Materials Science
  • Analytical Chemistry

Background:

  • Development of selective electrochemical sensors is crucial for clinical diagnostics.
  • Polypyrrole (PPY) is a conductive polymer with potential for sensor applications.
  • Chloride ion detection is important for monitoring physiological conditions.

Purpose of the Study:

  • To investigate the potential-response of a chloride-doped PPY microdisk electrode on a carbon fibre (CF).
  • To optimize polymerization conditions for enhanced electrode performance.
  • To evaluate the electrode's suitability for chloride detection in biological samples.

Main Methods:

  • Electrochemical synthesis of PPY films on CF substrates.
  • Cyclic voltammetry for electropolymerization and response characterization.
  • Optimization of pyrrole concentration, LiCl concentration, potential cycling range, and electropolymerization time.
  • Potentiometric measurements to determine Nernstian response and detection limit.
  • Application testing for chloride detection in serum samples.

Main Results:

  • Optimum polymerization conditions identified: cycling potential +0.8 to +1.0 V, 0.1-0.2M pyrrole, 0.1M LiCl, 15-20 min.
  • Achieved a Nernstian response of 56-58 mV/pCl.
  • Established a detection limit of 3.6 x 10^-5 M for chloride.
  • Demonstrated low resistance, short conditioning time, and fast response characteristics.
  • Successfully applied the electrode for chloride detection in serum.

Conclusions:

  • The chloride-doped PPY/CF microdisk electrode exhibits excellent potentiometric sensing capabilities for chloride ions.
  • Optimized preparation conditions lead to a highly sensitive and responsive sensor.
  • The developed electrode is a promising tool for the reliable detection of chloride in clinical serum samples.