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X-ray Diffraction of Biological Samples01:10

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X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...
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Discovery and Synthesis Optimization of Isoreticular Al(III) Phosphonate-Based Metal-Organic Framework Compounds Using High-Throughput Methods
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X-ray powder diffraction data for nine analgesics.

J E Kountourellis1, C K Markopoulou, F A Underwood

  • 1School of Pharmacy, 106, Aristotle University, Thessaloniki, 54006, Greece.

Talanta
|February 1, 1991
PubMed
Summary
This summary is machine-generated.

X-ray powder diffraction data were collected for nine analgesics using a diffractometer. Results were averaged and compared to powder camera films, noting intensity discrepancies.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Crystallography

Background:

  • Accurate characterization of pharmaceutical compounds is crucial for quality control.
  • X-ray powder diffraction (XRPD) is a standard technique for identifying crystalline solids.
  • Understanding variations in XRPD data due to sample preparation is important for reproducibility.

Purpose of the Study:

  • To obtain and present X-ray powder diffraction data for nine common analgesics.
  • To compare the results obtained using two different sample loading methods (McCreery and Byström-Asklund).
  • To evaluate potential discrepancies between diffractometer and powder camera film data.

Main Methods:

  • X-ray powder diffraction was performed on nine analgesic samples using a powder diffractometer.
  • Sample loading was conducted using both the McCreery and Byström-Asklund techniques.
  • Lattice spacings and relative line intensities were recorded and averaged.
  • Diffractometer data were compared with data obtained from powder camera films.

Main Results:

  • Lattice spacings and relative line intensities were tabulated for the nine analgesics.
  • Averaged data from the two loading methods provided a consistent dataset.
  • Discrepancies in line intensities were observed when comparing diffractometer and powder camera film data.
  • The study provides a reference dataset for the XRPD characteristics of these analgesics.

Conclusions:

  • The study successfully generated and tabulated XRPD data for nine analgesics.
  • The McCreery and Byström-Asklund sample loading methods yielded comparable results for lattice spacings and intensities.
  • Differences in intensity data between diffractometer and powder camera methods highlight the need for careful method selection and reporting in crystallographic analysis.