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Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
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Mass spectrometric approaches in structural identification of the reaction products arising from the interaction

A Lapolla1, C Gerhardinger, G Crepaldi

  • 1Instituto di Medicina Interna, Patologia Medica I, Policlinico Università di Padova, Italy.

Talanta
|April 1, 1991
PubMed

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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