Related Experiment Video
Updated: Jun 28, 2026

Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution
Published on: March 20, 2019
Flow injection fluorimetric determination of thiamine and copper based on the formation of thiochrome
T Perez-Ruiz1, C Martinez-Lozano, V Tomas
1Department of Analytical Chemistry, Faculty of Sciences, University of Murcia, Murcia, Spain.
Abstract:
The reaction, involving the oxidation of thiamine by copper(II) in basic solutions to fluorescent thiochrome, has been adapted to the determination of thiamine by flow-injection analysis. Linear calibration graphs are obtained between 0.30 and 6.02 mug/ml with a sampling rate of 50 samples/hr and a relative standard deviation of 0.53%. This reaction has also been adapted to the determination of copper(II) over the range 0.5-5.0 mug/ml. The applicability of both methods for determination of thiamine and copper is demonstrated by investigating the effect of potential interferences and by the analysis of real samples (pharmaceuticals for thiamine and ores and alloys for copper).
More Related Videos
06:53Magnetometric Characterization of Intermediates in the Solid-State Electrochemistry of Redox-Active Metal-Organic Frameworks
Published on: June 9, 2023
09:51TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples
Published on: September 19, 2025
Related Concept Videos
Precipitation Titration: Endpoint Detection Methods
In the Volhard method, a standard excess of AgNO3 is first added to the...
Electrophilic Aromatic Substitution: Fluorination and Iodination of Benzene