Related Experiment Video
Updated: Jun 28, 2026

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes
Published on: November 26, 2008
Thin-layer chromatography of laser dyes and dye analogs with cyclodextrins in the mobile phase
I R Politzer1, K T Crago, K Amos
1Department of Chemistry, Xavier University, New Orleans, Louisiana 70125, U.S.A.
Abstract:
The addition of beta-cyclodextrin and substituted hydroxyethyl and hydroxypropyl-beta-cyclodextrins to aqueous urea-containing mobile phases, can be used to enhance the migration of various laser dyes on thin layer chromatography. Selected laser dyes from the coumarin, rhodamine and bimane families were examined, as well as a number of dye analogs. Silica gel, polyamide and C18 reverse phase plates were utilized. Overall, the substituted hydroxypropyl-beta-cyclodextrins appeared to be the most effective for increasing dye migration.
More Related Videos
11:28Isolating and Incorporating Light-Harvesting Antennas from Diatom Cyclotella Meneghiniana in Liposomes with Thylakoid Lipids
Published on: August 28, 2018
08:54Determining Four Components in a Lipid Nanoparticle RNA Delivery System by Liquid Chromatography Combined with Evaporative Light Scattering Detector
Published on: May 30, 2025
Related Concept Videos
Thin-Layer Chromatography (TLC): Overview
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process
Chromatographic Methods: Classification
Chromatographic techniques are typically named by...
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
High-Performance Liquid Chromatography: Instrumentation