Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Potentiometry: Membrane Electrodes

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Single mode tapered fiber-optic interferometer based refractive index sensor and its application to protein sensing.

Optics express·2014

Same author

Molecularly imprinted polymers as optical sensing receptors: correlation between analytical signals and binding isotherms.

Analytica chimica acta·2011

Same author

Highly sensitive optical humidity probe.

Talanta·2008

Same author

Electronic structure of dinuclear gold(i) complexes.

Metal-based drugs·2008

Same author

A novel luminescent lifetime-based optrode for the detection of gaseous and dissolved oxygen utilising a mixed ormosil matrix containing ruthenium (4,7-diphenyl-1,10-phenanthroline)3Cl2 (Ru.dpp).

Analytical and bioanalytical chemistry·2006

Same author

Fluorescence sensor using a molecularly imprinted polymer as a recognition receptor for the detection of aluminium ions in aqueous media.

Analytical and bioanalytical chemistry·2006

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

A flow-cell optosensor for lead based on immobilized dithizone.

W A de Oliveira¹, R Narayanaswamy

¹Department of Instrumentation and Analytical Science, UMIST., P.O. Box 88, Manchester M60 1QD, U.K.

|November 1, 1992

Summary

This summary is machine-generated.

This study presents an optical sensor for lead detection using immobilized dithizone on XAD-4 resin. The sensor achieves a low detection limit of 1 x 10(-8)M, offering a reproducible and regenerable method for lead analysis.

More Related Videos

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

Published on: January 17, 2020

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Published on: June 1, 2012

Related Experiment Videos

Last Updated: Jun 28, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

Published on: January 17, 2020

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Published on: June 1, 2012

Area of Science:

Analytical Chemistry
Environmental Science
Materials Science

Background:

Accurate lead detection is crucial for environmental monitoring and public health.
Developing sensitive and selective sensors is an ongoing challenge in analytical chemistry.
Dithizone is a known chelating agent with potential for metal ion sensing.

Purpose of the Study:

To develop and evaluate an optical sensor for lead determination.
To investigate the performance of dithizone immobilized on XAD-4 resin as a sensor element.
To establish the detection limit, reproducibility, and regeneration capabilities of the sensor.

Main Methods:

Immobilization of dithizone onto XAD-4 resin.
Construction of an optical sensor utilizing the immobilized dithizone in a flow-cell.
Determination of lead concentrations in solution using spectrophotometry.
Evaluation of sensor response, reproducibility, and regeneration procedures.

Main Results:

The optical sensor successfully determined lead in the concentration range of 1 x 10(-5) to 3 x 10(-7)M.
A low detection limit of 1 x 10(-8)M (2 µg/l) for lead was achieved.
The method exhibited a standard deviation of 7% at a lead concentration of 1 x 10(-6)M.
The sensor demonstrated reproducible responses and effective regeneration using hydrochloric acid and citrate-hydroxylamine solutions.

Conclusions:

Dithizone immobilized on XAD-4 resin is a viable sensor element for optical lead detection.
The developed flow-cell based optical sensor offers high sensitivity and reliability for lead analysis.
The sensor's reproducibility and regenerability make it suitable for practical applications in lead monitoring.