HPLC analysis of thimerosal and its degradation products in ophthalmic solutions with electrochemical detection
J R Procopio1, M P da Silva, M Del Carmen Asensio
1Department of Analytical Chemistry and Instrumental Analysis, Autonoma University of Madrid, 28049 Madrid, Spain.
Abstract:
A reverse-phase high-performance liquid chromatographic assay using amperometric detection on a glassy-carbon electrode has been developed for analysis of thimerosal and its main degradation products, thiosalicylic acid and dithiodibenzoic acid, in ophthalmic formulations. A potential value of 0.9 V vs. Ag/AgCl was chosen for simultaneous detection of thimerosal and thiosalicylic acid, obtaining limits of detection of 1.0 and 0.2 ng injected, respectively. A potential value of 1.2V was applied for simultaneous determination of all three compounds studied, obtaining in this case limits of detection of 3,4 and 4 ng injected for thimerosal, thiosalicylic acid and dithiodibenzoic acid, respectively. The results obtained reveal the utility of the HPLC method in quality control of commercial products containing thimerosal with good detectability.
More Related Videos
05:03Quantitative Analysis of Dietary Vitamin A Metabolites in Murine Ocular and Non-Ocular Tissues Using High-Performance Liquid Chromatography
Published on: December 27, 2024
07:32Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System
Published on: January 30, 2019
