Sequential injection technique for automation of complex analytical procedures: fluorometric assay of factor thirteen

A non-segmented flow method is used to automate an analysis which involves five different reagents. The fluorometric assay for factor thirteen (FXIII) is performed by the sequential injection analysis (SIA) technique. Two reactions take place in a single fine SIA system to produce the final fluorescing product. Because of its mechanical simplicity and versatility, the sequential injection (SI) technique is shown to be an attractive tool for automation of a complex analytical procedure. In addition to collecting quantitative and kinetic information, the SI system is illustratively used for optimization of the analysis and for obtaining validation information.