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Simultaneous determination of warfarin and bromadiolone by derivative synchronous fluorescence spectrometry
S Panadero1, A Gómez-Hens, D Pérez-Bendito
1Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, E-14004 Córdoba, Spain.
Abstract:
By using second derivative synchronous fluorescence spectrometry the simple resolution of mixtures of the anticoagulant rodenticides warfarin and bromadiolone in the presence of beta-cyclodextrin is accomplished which causes a differential effect on the fluorescence intensity of these compounds. The determination method developed is simple, fast and inexpensive; in addition, measurements are performed in a single scan. Mixtures of warfarin and bromadiolone in ratios between 4:1 and 1:10 were satisfactorily resolved.
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