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Related Experiment Video

Updated: Jun 28, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
08:25

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Fluorimetric determination of nitrite.

N Jie1, J Yang, F Meng

  • 1Department of Chemistry, Shandong University, Jinan, People's Republic of China.

Talanta
|July 1, 1993
PubMed
Summary

A new fluorimetric method accurately determines nitrite using tryptophan in alkaline conditions. This rapid technique is effective for analyzing nitrite levels in water and food samples.

Area of Science:

  • Analytical Chemistry
  • Environmental Chemistry
  • Food Chemistry

Background:

  • Nitrite is an important analyte in environmental and food safety.
  • Accurate and rapid determination of nitrite is crucial for monitoring.
  • Existing methods may lack sensitivity or speed.

Purpose of the Study:

  • To develop a sensitive and rapid fluorimetric method for nitrite determination.
  • To utilize the reaction between nitrite and tryptophan for fluorescence generation.

Main Methods:

  • A fluorimetric assay was developed based on nitrite-tryptophan reaction.
  • The reaction was optimized in an alkaline medium.
  • The method was validated using water and food samples.

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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
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Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

Related Experiment Videos

Last Updated: Jun 28, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
08:25

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

Main Results:

  • The method demonstrated high sensitivity and rapidity for nitrite detection.
  • A highly fluorescent compound was formed from the reaction.
  • Successful application in real-world sample analysis (water and food).

Conclusions:

  • The described fluorimetric method offers a reliable approach for nitrite quantification.
  • This technique is suitable for routine analysis in various matrices.
  • It provides a valuable tool for environmental and food safety assessments.