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Updated: Jun 28, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
Published on: September 19, 2018
Potentiometric monitoring of proteins--part 6. Indirect potentiometry
1Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, U.K.
Abstract:
In a study of the indirect potentiometry of amino acids and proteins with metal ions it has been discovered that electrode potential responses to organic ligand concentrations can be linear over a range of tens of millivolts. In this paper the reasons for this are considered and a general model is presented in terms of an electrode of the second kind. Experimental results for the indirect potentiometry of a number of amino acids and proteins are then presented and are discussed in the context of the model derived. Applications to flow injection analysis and to the determination and identification of proteins eluting from a gel chromatography column are also considered.
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