Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Alpha band disruption in the AD-continuum starts in the Subjective Cognitive Decline stage: a MEG study.

Scientific reports·2016

Same author

Development of a thiourea derivative polymer combined to a Direct Mercury Analyser for screening and pre-concentration of mercury species.

Talanta·2016

Same author

Silver speciation and characterization of nanoparticles released from plastic food containers by single particle ICPMS.

Talanta·2016

Same author

Bioconcentration of ionic cadmium and cadmium selenide quantum dots in zebrafish larvae.

Chemosphere·2016

Same author

A new specific polymeric material for mercury speciation: Application to environmental and food samples.

Analytica chimica acta·2015

Same author

Zebrafish (Danio rerio) eleutheroembryo-based procedure for assessing bioaccumulation.

Environmental science & technology·2015

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

Published on: September 19, 2025

A biosensor for ferric ion.

J M Barrero¹, M C Morino-Bondi, M C Pérez-Conde

¹Department of Analytical Chemistry, Faculty of Chemistry, Complutense University, 28040 Madrid, Spain.

|November 1, 1993

Summary

A novel biosensor utilizing pyoverdin effectively monitors iron(III) in water samples. This stable, regenerated sensor offers sensitive and precise iron detection, comparable to ICP-AES methods.

Area of Science:

Analytical Chemistry
Environmental Science
Biotechnology

Background:

Accurate monitoring of iron in water is crucial for environmental and health assessments.
Existing methods for iron detection can be complex or lack selectivity.
Development of rapid, selective, and stable biosensors is an ongoing area of research.

Purpose of the Study:

To develop and characterize a new biosensor for selective iron(III) monitoring.
To evaluate the analytical performance and stability of the developed biosensor.
To assess the applicability of the biosensor for real-world water sample analysis.

Main Methods:

Immobilization of pyoverdin, a fluorescent pigment from Pseudomonas fluorescens, onto controlled pore glass (CPG).
Development of a flow-through cell biosensor for iron(III) detection.

More Related Videos

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Published on: March 12, 2015

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))

Published on: May 4, 2020

Related Experiment Videos

Last Updated: Jun 28, 2026

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

Published on: September 19, 2025

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Published on: March 12, 2015

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))

Published on: May 4, 2020

Regeneration of the sensor using 1M HCl.

Determination of analytical characteristics including detection limit, precision, and linear range.

Comparison with Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES).

Main Results:

The biosensor demonstrated high selectivity for iron(III).
Detection limits were 10 ng/ml in solution and 3 ng/ml in immobilized form.
The sensor showed good stability, usable for over 3 months (>1000 determinations).
Regeneration was rapid (approx. 2 min).
No significant difference was observed compared to ICP-AES for water samples.

Conclusions:

The developed pyoverdin-based biosensor is a sensitive, selective, and stable tool for iron(III) monitoring.
The biosensor offers a viable alternative to conventional methods like ICP-AES for water analysis.
Its ease of regeneration and long-term stability make it suitable for continuous monitoring applications.