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Updated: Jun 28, 2026

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Published on: April 5, 2019
Retention in analytical ion exchange chromatography-1. Strong electrolytes on strong ion exchangers
G O Fóti1, P Hajós, E S Kováts
1Laboratoire de Chimie Technique de l'Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
Abstract:
A retention equation of general validity is adapted to ion exchange chromatography providing a self-consistent interpretation of retention of solutes and system peaks. Discussion is limited to eluent mixtures containing ions of invariant charge and to stationary phases with pH-independent surface charge. An interpretation is proposed for the retention volume of system peaks in binary eluents (mixtures of two eluent counter-ions). Retention volumes of labelled counter-ions of the eluent appear to provide useful information about the chromatographic system with clear interpretation in any multicomponent eluent.
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