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Updated: Jun 28, 2026
08:25
Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Flow injection spectrophotometric determination of nitrite
1Department of Chemistry, Rani Durgavati University, Jabalpur, Madhya Pradesh, India.
Talanta
|August 1, 1994
Summary
A new method uses 4-nitroaniline to detect nitrite (NO2-N) in water. This simple, one-step procedure offers a low detection limit and is unaffected by common interfering ions.
Area of Science:
- Analytical Chemistry
- Environmental Chemistry
Background:
- 4-Nitroaniline forms a yellow solution in acidic conditions.
- Nitrite reacts rapidly with 4-nitroaniline in an acidic medium.
Purpose of the Study:
- To develop a new, simple, and rapid method for determining nitrite concentrations.
- To identify the reaction product between 4-nitroaniline and nitrite.
- To assess the method's applicability in natural water samples.
Main Methods:
- Reversed flow injection analysis.
- Spectrophotometric measurement of color intensity decrease.
- Reaction of 4-nitroaniline with nitrite in an acidic medium.
Main Results:
- The reaction yields a colorless product, identified as 4-nitrophenyl diazo cation.
- The method allows for the determination of nitrite in the range of 5 µg-5 mg/L.
- The limit of detection is as low as 2 µg/L nitrite nitrogen (NO2-N).
- Common interfering ions like copper(II) and lead(II) do not affect the results.
Conclusions:
- The developed method is a sensitive and selective approach for nitrite determination.
- The method is robust and suitable for analyzing nitrite in natural waters with good precision (1.8-3.5% RSD).
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