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Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...

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Updated: Jun 28, 2026

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
11:05

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Published on: October 26, 2016

Capillary electrophoresis.

M L Marina1, M Torre

  • 1Departamento de Química Analítica, Facultad de Ciencias, Universidad de Alcalá de Henares, 28871 Alcalá de Henares, Madrid, Spain.

Talanta
|September 1, 1994
PubMed
Summary
This summary is machine-generated.

This study explores capillary electrophoresis (CE), detailing its core principles, essential instrumentation, and key applications. Understanding CE is crucial for various analytical chemistry fields.

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Sheathless Capillary Electrophoresis&#8211;Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

Related Experiment Videos

Last Updated: Jun 28, 2026

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
11:05

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Published on: October 26, 2016

Sheathless Capillary Electrophoresis&#8211;Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Capillary electrophoresis (CE) is a powerful separation technique.
  • Its versatility makes it applicable across diverse scientific disciplines.

Purpose of the Study:

  • To elucidate the fundamental characteristics of capillary electrophoresis.
  • To provide a comprehensive overview of CE principles and instrumentation.

Main Methods:

  • Review of established capillary electrophoresis principles.
  • Description of typical CE instrumentation.
  • Analysis of prominent CE applications.

Main Results:

  • Detailed explanation of the electrophoretic separation mechanism in capillaries.
  • Overview of key components: power supply, capillary, detector, and autosampler.
  • Highlighting applications in pharmaceutical analysis, environmental monitoring, and biochemical research.

Conclusions:

  • Capillary electrophoresis offers high-efficiency separations.
  • The described principles and instrumentation are foundational for CE practitioners.
  • CE remains a vital tool with broad applicability in modern analytical science.