Related Experiment Video
Updated: Jun 28, 2026

Determination of the Photoisomerization Quantum Yield of a Hydrazone Photoswitch
Published on: February 7, 2022
Fluorimetric determination of phenothiazine derivatives by photooxidation in a flow-injection system
B Laassis1, J J Aaron, M C Mahedero
1Institut de Topologie et de Dynamique des Systèmes de l'Université Paris 7, associé an CNRS, URA 34, 1 rue Guy de la Brosse, Paris, France.
Abstract:
Flow-injection analysis (FIA) was combined with photochemically induced fluorescence (PF) detection for the determination of four phenothiazine derivatives, including unsubstituted phenothiazine, thionine, Azure A and Methylene Blue. The working analytical parameters (flow-rate, injected volume, photoreactor length) were optimized. Linear calibration graphs were obtained over about two orders of magnitude, with relative standard deviation within the range 1-2.3%. Limits of detection were between 13 and 35 ng/ml, according to the compound. The FIA-PF method was applied to the determination of phenothiazines in urine samples. Mean recoveries ranged from 94 to 117%.
More Related Videos
Related Concept Videos
Flame Photometry: Lab
Gas Chromatography: Types of Detectors-II
Photoluminescence: Applications
Fast Reactions
Fluorescence and Phosphorescence: Instrumentation
Flame Photometry: Overview

