A simplified enzyme-based fiber optic sensor for hydrogen peroxide and oxidase substrates

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biosensors

Background:

• Hydrogen peroxide (H2O2) is a key analyte in various biological processes and industrial applications.
• Accurate and sensitive detection of H2O2 is crucial for diagnostics and quality control.
• Existing methods for H2O2 detection can be complex or lack selectivity.

Purpose of the Study:

• To develop a simplified, enzyme-based fiber optic sensor for selective hydrogen peroxide determination.
• To utilize a novel fluorimetric substrate, thiamine, for indicating the sensing process.
• To explore the sensor's applicability in multi-analyte detection systems.

Main Methods:

• Immobilization of horseradish peroxidase (HRP) onto a bovine albumin matrix using glutaraldehyde.
• Development of a fiber optic sensor system employing thiamine as a fluorimetric substrate.
• Optimization of reaction conditions for sensor performance.
• Co-immobilization of oxidase enzymes with HRP for multi-analyte sensing.

Main Results:

• The developed sensor system demonstrated selective determination of hydrogen peroxide.
• Optimized conditions yielded a measuring range up to 1 x 10(-4)M H2O2.
• A low limit of detection of 5 x 10(-7)M H2O2 was achieved within a 5-minute response time.
• The sensor system was successfully adapted for the determination of uric acid, D-amino acid, L-amino acid, glucose, cholesterol, choline, and acetylcholine.

Conclusions:

• A simplified and effective enzyme-based fiber optic sensor for hydrogen peroxide detection has been successfully developed.
• The sensor exhibits high sensitivity, selectivity, and rapid response time.
• The system's adaptability for multi-analyte determination highlights its potential for broad biochemical sensing applications.