Related Experiment Video
Updated: Jun 28, 2026

Laboratory Simulation of an Iron(II)-rich Precambrian Marine Upwelling System to Explore the Growth of Photosynthetic Bacteria
Published on: July 24, 2016
Spectrophotometric determination of H(2)O(2) in marine waters with leuco crystal violet
1Department of Oceanography, Old Dominion University, Norfolk, VA 23529-0276, U.S.A.
Abstract:
H(2)O(2) in marine waters may be determined by measuring the absorbance of crystal violet (CV(+)), formed by the oxidation of leuco crystal violet (LCV) by H(2)O(2) in the presence of the enzyme horseradish peroxidase, at 592 nm at pH 4. The detection limit and the precision of the method are about 0.02 muM and +/- 1% (at a concentration of 0.03 muM), respectively. The results obtained by this method and by a widely used fluorimetric method agreed with each other well. After color development, samples together with the sub-samples for constructing the calibration curve may be stored for up to 5 days before their absorbances are measured without significantly changing the estimated concentration of H(2)O(2).
Related Concept Videos
Spectrophotometry: Introduction
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
UV–Vis Spectrometers
UV–Vis Spectroscopy: Beer–Lambert Law
UV–Vis Spectroscopy: Woodward–Fieser Rules
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview
UV–Vis Spectroscopy of Conjugated Systems
One of the factors influencing λmax is the extent of conjugation in the...

