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UV–Vis Spectrometers

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

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Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

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Fluorescence and Phosphorescence: Instrumentation

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There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...

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Related Experiment Video

Updated: Jun 28, 2026

Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

Published on: September 27, 2019

A multi-wavelength photometer based on light-emitting diodes.

P C Hauser¹, T W Rupasinghe, N E Cates

¹The University of Auckland, Department of Chemistry, Auckland, New Zealand.

|April 1, 1995

Summary

A new multi-wavelength photometer uses light-emitting diodes and photodiodes for direct absorbance readings. This cost-effective instrument can replace conventional spectrophotometers for many photometric analyses.

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Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

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Area of Science:

Analytical Chemistry
Optical Instrumentation

Background:

Spectrophotometers are standard tools for absorbance measurements.
Conventional spectrophotometers can be expensive and bulky.

Purpose of the Study:

To develop an inexpensive and compact multi-wavelength photometer.
To evaluate its performance as a spectrophotometer alternative.

Main Methods:

Utilizing seven different colored light-emitting diodes (LEDs) coupled via fiber optics.
Employing photodiodes and a log-ratio amplifier for direct absorbance readings.
Using optical filters to narrow emission bands from blue LEDs.

Main Results:

An affordable, compact multi-wavelength photometer was successfully constructed.
The photometer demonstrated effective absorbance measurements across the visible spectrum.
Performance was comparable to conventional spectrophotometers for common analytical procedures.

Conclusions:

The developed LED-based photometer offers a viable, cost-effective alternative to traditional spectrophotometers.
This instrument is suitable for various photometric analytical applications.
Further validation across diverse analytical methods is recommended.