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Updated: Jun 28, 2026

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
Published on: October 21, 2016
New fluorigenic reagents and their fluorescent reactions for amino acid measurements
1Department of Chemistry, Loughborough University of Technology, Loughborough, Leicestershire, U.K.
Abstract:
Naphthalene-2,3-dicarboxaldehyde, 1-phenylnaphthalene-2,3-dicarboxaldehyde and anthracene-2,3-dicarboxaldehyde have been characterized for use as fluorigenic reagents for the determination of primary amines and amino acids at the microgram level. The reaction conditions, spectral properties and stability of the derivatives (isoindoles) have been investigated with standard amino acids. The results have been compared with other fluorigenic reagents such as ortho-phthalaldehyde.
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