Sample illumination configurations for spatially resolved Raman spectrometry using a charge-coupled device detector.

Area of Science:

• Spectroscopy
• Analytical Chemistry
• Materials Science

Background:

• Obtaining spatial and spectral information from complex samples is challenging.
• Effective sample illumination is critical for accurate data acquisition.
• Charge-coupled device (CCD) detectors offer high sensitivity for spectroscopic analysis.

Purpose of the Study:

• To present two straightforward sample illumination configurations for CCD detectors.
• To showcase the utility of these methods for analyzing complex and heterogeneous samples.
• To demonstrate the capability for simultaneous spectral acquisition and spatial resolution.

Main Methods:

• Utilizing vertical sample illumination for simultaneous Raman spectra acquisition.
• Employing horizontal multiline sample illumination with different lasers (488 nm and 632.8 nm).
• Dividing the CCD detector into regions for spatially resolved fluorescence spectra.

Main Results:

• Simultaneous Raman spectra of a three-compound heterogeneous sample were obtained.
• Spatially resolved resonant Raman and surface-enhanced resonant Raman spectra of Ni-PAPH were observed with low integration time.
• Spatially resolved fluorescence spectra of a homogeneous dye mixture were acquired in 1 second.

Conclusions:

• Simple CCD illumination configurations are effective for complex sample analysis.
• These techniques allow for simultaneous spectral acquisition and spatial resolution.
• Rapid analysis of heterogeneous and homogeneous samples is achievable.