Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Electrodeposition01:08

Electrodeposition

Electrodeposition is a technique used to separate an analyte from interferents by electrochemical processes. Here, the analyte is a metal ion that can be deposited on an electrode immersed in the sample solution. The electrochemical setup consists of an anode and a cathode. When an electric current is applied to the setup, oxidation occurs at the anode. At the cathode, which consists of a large metal surface, metal ions undergo reduction and deposit onto the surface.
Electrodeposition can...
Depolarizing Blockers: Pharmocokinetics01:19

Depolarizing Blockers: Pharmocokinetics

Depolarizing blockers are administered through intravenous injection. Succinylcholine is the most common choice of depolarizing blockers in emergency clinical practices. Although they have a rapid onset, they readily diffuse away from the motor end plate into the extracellular fluid. They are metabolized by enzymes such as liver butyrylcholinesterase and plasma pseudocholinesterases. This produces a short duration of action, typically 5-10 minutes long, unlike nondepolarizing blockers, which...
Cellular Membranes and Drug Transport01:24

Cellular Membranes and Drug Transport

Drugs must traverse multiple biological barriers, such as multi-layered skin, single-layered intestinal epithelium, and the plasma membrane, to reach their target sites within the body. The plasma membrane, a highly structured composite of phospholipids, carbohydrates, and proteins, is the cell's protective boundary, facilitating selective substance exchange.
Phospholipids arrange themselves into a bilayer, with hydrophilic heads oriented outward and hydrophobic tails facing inward.
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Antiepileptic Drugs: Potassium Channel Activators01:20

Antiepileptic Drugs: Potassium Channel Activators

Ezocgabine or retigabine, an antiepileptic drug of remarkable efficacy, has revolutionized the management of seizures. It is a potassium channel activator, explicitly targeting the family of Q subtype potassium channels. It enhances the transmembrane potassium currents, regulating neuronal excitability. This action stabilizes the resting membrane potential, a pivotal factor in mitigating the hyperexcitability that characterizes epilepsy.
Ezogabine has gained approval as an adjunctive treatment...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polarity-based fractionation in proteomics: hydrophilic interaction vs reversed-phase liquid chromatography.

Biomedical chromatography : BMC·2015
Same author

Freeze-dried amorphous dispersions for solubility enhancement of thermosensitive API having low molecular lipophilicity.

Drug research·2014
Same author

Pharmacokinetics and cardiovascular effect of etoricoxib in the absence or presence of St. John's Wort in rats.

Arzneimittel-Forschung·2012
Same author

A new construction for a potentiometric, enantioselective membrane electrode--its utilization to the S-captopril assay.

Talanta·2008
Same author

Simultaneous flow injection determination of calcium and fluoride in natural and borehole water with conventional ion-selective electrodes in series.

Talanta·2008
Same author

Simultaneous detection of S and R captopril using sequential injection analysis.

Talanta·2008

Related Experiment Video

Updated: Jun 28, 2026

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
11:44

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Published on: January 19, 2022

Moclobemide-selective membrane electrode and its pharmaceutical applications.

R I Stefan1, G E Baiulescu, H Y Aboul-Enein

  • 1Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, Blvd. Republicii # 13, 70346, Bucharest-3, Romania.

Talanta
|July 1, 1996
PubMed
Summary

A novel liquid membrane electrode was developed for moclobemide analysis. This electrode offers a sensitive and selective method for determining moclobemide in pharmaceutical formulations and active substances.

More Related Videos

In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays
10:05

In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays

Published on: September 20, 2021

Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes
07:45

Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes

Published on: August 16, 2018

Related Experiment Videos

Last Updated: Jun 28, 2026

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
11:44

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Published on: January 19, 2022

In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays
10:05

In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays

Published on: September 20, 2021

Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes
07:45

Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes

Published on: August 16, 2018

Area of Science:

  • Analytical Chemistry
  • Electrochemistry
  • Pharmaceutical Analysis

Background:

  • Moclobemide analysis requires sensitive and selective methods.
  • Existing methods may have limitations in speed or applicability.
  • Development of new potentiometric sensors is crucial for pharmaceutical quality control.

Purpose of the Study:

  • To develop and characterize a liquid membrane electrode for moclobemide determination.
  • To evaluate the electrode's analytical performance, including linearity, detection limit, and selectivity.
  • To assess the electrode's applicability in direct and indirect potentiometric methods for drug analysis and dissolution studies.

Main Methods:

  • Preparation of a liquid membrane electrode using a moclobemide-dipicrylamine ion-pair complex in nitrobenzene.
  • Potentiometric measurements to determine linearity, slope, and detection limit across a specified pH range.
  • Selectivity testing against potential interfering ions and common pharmaceutical excipients.
  • Application of the electrode in direct and indirect potentiometric assays for moclobemide in active substance and drug formulations.
  • Numerical simulation of physical processes for dissolution rate determination.

Main Results:

  • The electrode exhibited a linear response for moclobemide from 10(-3) to 10(-6) M with a slope of 50.7 mV/decade (pH 3.5-8).
  • A detection limit of 3 x 10(-7) M was achieved, with good stability, reproducibility, and fast response times.
  • The electrode demonstrated good selectivity, with minimal interference from common excipients, though mianserin and thiamine showed some interference.
  • Indirect potentiometric determination of moclobemide yielded high average recovery (99.96%) and low relative standard deviation (<2% in drugs).
  • The electrode was successfully applied to determine the dissolution rate of moclobemide tablets.

Conclusions:

  • The developed liquid membrane electrode is a robust and reliable tool for the potentiometric determination of moclobemide.
  • The electrode's performance characteristics make it suitable for quality control of moclobemide active substance and pharmaceutical products.
  • The method is effective for assessing drug dissolution rates, contributing to pharmaceutical development and quality assurance.