Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Color Vision01:24

Color Vision

Color perception begins in the retina, the light-sensitive layer at the back of the eye. Two main theories explain how colors are seen: the trichromatic theory and the opponent-process theory. The trichromatic theory, proposed by Thomas Young in 1802 and extended by Hermann von Helmholtz in 1852, suggests that color vision is based on three types of cone receptors in the retina. These cones are sensitive to different but overlapping ranges of wavelengths corresponding to red, blue, and green.
Titrimetric Methods: Types and Commonly Used Strategies01:08

Titrimetric Methods: Types and Commonly Used Strategies

In chemistry, titrimetric methods are broadly classified into three types: volumetric, gravimetric, and coulometric. Volumetric titrations involve measuring the volume of a titrant of known concentration that is required to react completely with an analyte. In gravimetric titrations, the standard solution reacts with the analyte to form an insoluble precipitate, which is filtered, dried, and weighed. In coulometric titrations, current is applied to an electrochemical reaction until the reaction...
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Photoreceptors and Visual Pathways01:22

Photoreceptors and Visual Pathways

At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category, whereas...
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of colour quality of complexometric indicators in the titration of Nickel(II) with EDTA by tristimulus colorimetry.

Talanta·1993
Same author

Specification of colour changes of metallochromic indicators in the titration of bismuth with EDTA.

Talanta·1991
See all related articles

Related Experiment Video

Updated: Jun 28, 2026

ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis
07:11

ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis

Published on: August 19, 2021

Basic aspects and applications of tristimulus colorimetry.

K M Krishna Prasad, S Raheem, P Vijayalekshmi

    Talanta
    |August 1, 1996
    PubMed
    Summary
    This summary is machine-generated.

    This study details using Commission International de L

    More Related Videos

    Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters
    07:05

    Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters

    Published on: June 18, 2021

    Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
    09:46

    Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

    Published on: August 19, 2013

    Related Experiment Videos

    Last Updated: Jun 28, 2026

    ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis
    07:11

    ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis

    Published on: August 19, 2021

    Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters
    07:05

    Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters

    Published on: June 18, 2021

    Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
    09:46

    Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

    Published on: August 19, 2013

    Area of Science:

    • Analytical Chemistry
    • Colorimetry
    • Spectrophotometry

    Background:

    • Accurate color specification is crucial for scientific measurements.
    • Traditional methods for evaluating indicator color changes have limitations.

    Purpose of the Study:

    • To review color specification methods using CIE tristimulus coordinates and color spaces.
    • To evaluate quantitative parameters for assessing indicator color changes.
    • To discuss screened indicators and computational tools for color analysis.

    Main Methods:

    • Review of 1931 Commission International de L'Eclairage (CIE) tristimulus coordinates.
    • Analysis of color spaces for small color difference measurement.
    • Evaluation of quantitative parameters for acid-base and complexometric indicators.
    • Discussion on screened indicators and computer programs for parameter calculation.

    Main Results:

    • Established methods for precise color specification using CIE coordinates.
    • Identified quantitative parameters for evaluating indicator color changes.
    • Highlighted the role of screened indicators in improving endpoint detection.
    • Reviewed various algorithms and computer programs for colorimetric calculations.

    Conclusions:

    • Quantitative color measurement enhances the precision of indicator-based titrations.
    • Screened indicators offer improved visual endpoints.
    • Computational tools facilitate accurate color parameter analysis.