Related Experiment Video
Updated: Jun 28, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Enhanced decompositions of some cyano complexes during diode array spectrophotometric measurements
G López-Cueto1, J F Rodríguez-Medina, C Ubide
1Departamento de Química Analítica, Facultad de Ciencias, Universidad de Alicante, Apdo. 99, 03080 Alicante, Spain.
Abstract:
Acidic solutions of the Fe(III), Mo(V), W(V) and Mn(IV) cyano complexes are known to decompose photochemically. These reactions do not take place to an appreciable extent when the cyano complexes are subjected to spectrophotometric measurements by means of conventional spectrophotometers, but they do take place when diode array spectrophotometers are used. The reactions are autoaccelerated, at least in the cases of Mo(V), W(V) and Mn(IV), and consist of photoinduced reductions of the metal ion. This effect should be taken into account in all cases where the measured signal has to be used for quantitative purposes, but its significance must be emphasized mainly in the field of kinetic analysis, where it can lead to large errors. The extent of these reactions, and therefore the size of the errors, can be lowered by using UV cut-off filters and by taking discontinuous measurements with suitable integration times. All these possibilities, which are of general application, are discussed.
More Related Videos
10:21Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions
Published on: October 5, 2019
12:51A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
Published on: November 14, 2015
Related Concept Videos
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
UV–Vis Spectrometers
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
UV–Vis Spectroscopy of Conjugated Systems
One of the factors influencing λmax is the extent of conjugation in the...
Aryldiazonium Salts to Azo Dyes: Diazo Coupling