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Assay of cisapride in pharmaceutical formulations by extraction spectrophotometry
C S Sastry1, Y Srinivas, P V Rao
1Department of Organic Chemistry, Foods, Drugs and Water, Andhra University, Visakhapatnam-530 003, India.
Abstract:
Four simple and sensitive spectrophotometric methods (A-D) for the assay of cisapride in pure and dosage forms based on the formation of chloroform soluble ion-associates under specified experimental conditions are described. Four acidic dyes, namely, Suprachen Violet 3B (SV 3B, method A), Erioglaucine A (EG-A, method B), Naphthalene Blue 12 BR (NB-12BR, method C) and Tropaeolin 000 (TP 000, method D) are utilized. The extracts of the ion-associates exhibit absorption maxima at 595, 640, 620 and 500 nm for methods A, B, C and D, respectively. Beer's law and the precision and accuracy of the methods are checked by the UV reference method. The results are reproducible with an accuracy of +/-1.0%. The methods are found to be suitable for the determination of cisapride in the presence of the other ingredients that are usually present in dosage forms.
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