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Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material
M Khayyami1, M T Pérez Pita, N Peña Garcia
1Department of Pure and Applied Biochemistry, Centre for Chemistry and Chemical Engineering, Lund University, S-221 00 Lund, Sweden.
Abstract:
A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon-10(-9) mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.
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