Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Fluorescence and Phosphorescence: Instrumentation

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Treatment of vancomycin-intermediate Staphylcoccus aureus endocarditis with linezolid.

Scandinavian journal of infectious diseases·2004

Same author

Laparoscopic repair of various types of biliary-enteric fistula: three cases.

Surgical endoscopy·2004

Same author

Hydrocele of the canal of Nuck in a girl: ultrasound and MR appearance.

The British journal of radiology·2004

Same author

Steady-state solutions of hydrodynamic traffic models.

Physical review. E, Statistical, nonlinear, and soft matter physics·2004

Same author

Common genetic polymorphisms in the promoter of resistin gene are major determinants of plasma resistin concentrations in humans.

Diabetologia·2004

Same author

Multifactor-dimensionality reduction shows a two-locus interaction associated with Type 2 diabetes mellitus.

Diabetologia·2004

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Fluorescence detection in short capillary and chip using a variable wavelength epi-fluorescence microscope.

Y Zhang¹, H K Lee, S F Li

¹Department of Chemistry, National University of Singapore, Kent Ridge Crescent, Singapore 119260, Singapore.

|October 31, 2008

Summary

This study presents rapid and efficient separation techniques for five free acid forms of porphyrins using capillary electrophoresis and microchip electrophoresis. Both methods achieved high-resolution separations in under 5 minutes.

More Related Videos

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Related Experiment Videos

Last Updated: Jun 28, 2026

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Area of Science:

Analytical Chemistry
Separation Science

Background:

Porphyrins are vital biological molecules, and their separation is crucial for research.
Existing separation methods can be time-consuming and less efficient.

Purpose of the Study:

To develop and compare fast, efficient separation methods for free acid porphyrins.
To evaluate the performance of capillary electrophoresis (CE) and microchip electrophoresis (ME) for porphyrin analysis.

Main Methods:

Capillary electrophoresis (CE) was performed in a short capillary (6 cm) with an electric field strength of 214 V cm(-1).
Microchip electrophoresis (ME) was conducted on a custom-fabricated glass microchip with a separation length of 2.8 cm and an electric field strength of 500 V cm(-1).
A variable wavelength epi-fluorescence microscope served as the on-column detector for both techniques.

Main Results:

CE achieved baseline resolution of five free acid porphyrins within 5 minutes.
ME achieved baseline resolution in less than 2 minutes, demonstrating superior speed.
Both methods proved effective for the rapid separation of porphyrin isomers.

Conclusions:

Microchip electrophoresis offers a significantly faster and highly efficient alternative for porphyrin separation compared to conventional capillary electrophoresis.
These developed methods provide valuable tools for the rapid analysis of porphyrins in various scientific applications.