Related Experiment Video
Updated: Jun 28, 2026

Analysis of Organochlorine Pesticides in a Soil Sample by a Modified QuEChERS Approach Using Ammonium Formate
Published on: January 20, 2023
Quantitative analysis of pesticides by capillary column high performance liquid chromatography combined with
1Department of Chemistry, National University of Singapore, Kent Ridge, Singapore 119260, Singapore.
Abstract:
High performance liquid chromatography (HPLC) combined with solid-phase extraction was reported on, for simultaneous analysis of pesticides in this work. The separation of 12 pesticides was achieved on a C(18) capillary column with gradient elution. Sub-microlitre injection volume of the samples and a U-shaped 35 nl flow cell were used to improve the separation and detection. In addition, the method used C(18) solid-phase extraction disks to allow a 250-fold enrichment of the pesticides from fortified water and apple samples. The calculated detection limits range was 0.15-0.8 mug/l. Under the optimal extraction conditions, recoveries of 85-107% for most of the pesticides at 1.0-10.0 mug/l level, were obtained.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Electrophoresis: Overview
There...
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Types of Detectors
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

