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Updated: Jun 28, 2026

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
Published on: May 16, 2019
Direct determination of copper and zinc in cow milk, human milk and infant formula samples using electrothermal
N Campillo1, P Viñas, I López-García
1Dpto. de Quimica Analitica, Facultad de Quimica, Universidad de Murcia, E-30071 Murcia, Spain.
Abstract:
Infant formula and powdered milk samples were suspended in a medium containing 20% ethanol, 0.65% nitric acid, 6% hydrogen peroxide and 0.1% ammonium dihydrogenphosphate and directly introduced in the electrothermal atomizer. Liquid milk samples were diluted with this suspension solution. The build-up of carbonaceous residues inside the atomizer was avoided by using a combination of hydrogen peroxide and nitric acid. Fast programs were used to simplify the heating cycles. Calibration was carried out using aqueous standards and the detection limits for copper and zinc were found to be 70 and 15 pg, respectively. The metal contents of several milk samples obtained by direct introduction of the samples agree with those obtained by means of a conventional procedure based on the total dissolution of the samples. The reliability of the methods was also confirmed by analyzing three certified reference materials. The free and protein binding fractions were measured by ultrafiltration membrane permeability of the milk samples.
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