A selective voltammetric method for uric acid detection at Nafion(R)-coated carbon paste electrodes
This study presents a selective method for determining uric acid, even with high ascorbic acid levels. The technique uses a Nafion-coated carbon paste electrode and a specific electrolysis potential to achieve accurate uric acid measurements.
Area of Science:
- Analytical Chemistry
- Electrochemistry
- Biomedical Science
Background:
- Accurate determination of uric acid is crucial for diagnosing conditions like gout and kidney disease.
- High concentrations of ascorbic acid (Vitamin C) in biological samples interfere with uric acid measurements.
- Existing methods often require complex sample preparation to remove interfering substances.
Purpose of the Study:
- To develop a selective and sensitive square-wave voltammetric method for uric acid determination.
- To overcome the interference from high concentrations of ascorbic acid.
- To enable direct measurement of uric acid in biological samples like human urine.
Main Methods:
- Utilized a Nafion(R)-coated carbon paste electrode for electrochemical analysis.
- Employed square-wave voltammetry for sensitive detection.
- Implemented a selective pre-electrolysis step at +0.4 V vs. Ag/AgCl to oxidize interfering ascorbic acid.
Main Results:
- Achieved high selectivity for uric acid in the presence of up to 1.5 mM ascorbic acid.
- Established a linear calibration curve for uric acid from 0-50 muM with a correlation coefficient of 0.9984.
- Determined a low detection limit of 0.25 muM for uric acid.
- Demonstrated successful application in the direct analysis of uric acid in human urine samples.
Conclusions:
- The developed method offers a simple, selective, and sensitive approach for uric acid quantification.
- The use of a Nafion(R)-coated electrode combined with controlled potential electrolysis effectively eliminates ascorbic acid interference.
- This method holds significant potential for routine clinical diagnostics and biological research without extensive sample pretreatment.
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