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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Fluorescence optical fiber sensor for tetracycline.

Y Wang¹, W H Liu, K M Wang

¹College of Chemistry and Chemical Engineering, Southwest China Normal University, Chongqing 400715, People's Republic of China; College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People's Republic of China.

|October 31, 2008

Summary

A novel optical fiber sensor effectively monitors tetracycline using fluorescence quenching. This reversible and reproducible sensor shows high selectivity and rapid response for pharmaceutical analysis.

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Last Updated: Jun 28, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

Area of Science:

Analytical Chemistry
Materials Science
Biomedical Engineering

Background:

Tetracycline antibiotics are widely used but require sensitive monitoring.
Existing tetracycline detection methods can be complex or lack selectivity.
Development of rapid, selective, and reversible sensors is crucial for pharmaceutical quality control.

Purpose of the Study:

To develop a novel optical fiber sensor for the selective and sensitive detection of tetracycline.
To investigate the sensor's performance characteristics, including response range, limit of detection, response time, and reversibility.
To evaluate the sensor's applicability in analyzing tetracycline in pharmaceutical preparations.

Main Methods:

Fabrication of an optical fiber sensor incorporating a polymer film with 1,4-bis(5,5'-dimethylbenzoxazole-1',3'-yl-2')benzene.
Utilizing fluorescence quenching mechanism for tetracycline detection.
Extraction of tetracycline from aqueous phase into the polymer film.
Characterization of sensor response, selectivity, and reproducibility.

Main Results:

The sensor demonstrated a linear response to tetracycline over a concentration range of 6.98x10(-7) to 8.73x10(-5) mol l(-1).
A low detection limit of 1.06x10(-7) mol l(-1) and a rapid response time (<30 s) were achieved.
The sensor exhibited high selectivity for tetracycline, with significant discrimination against common pharmaceutical agents and salts.
High recovery rates (95.3-98.3%) were obtained when analyzing tetracycline in commercial pharmaceutical formulations.

Conclusions:

The developed optical fiber sensor offers a promising platform for accurate and efficient tetracycline monitoring.
The sensor's reversibility, reproducibility, selectivity, and rapid response make it suitable for real-world pharmaceutical analysis.
This technology can contribute to improved quality control and safety of tetracycline-containing drugs.