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Updated: Jun 28, 2026

A Colorimetric Method for Measuring Iron Content in Plants
Published on: September 7, 2018
Simultaneous determination of iron, cobalt, nickel and copper by UV-visible spectrophotometry with multivariate
A M Garcia Rodriguez1, A Garcia de Torres, J M Cano Pavon
1Department of Analytical Chemistry, Faculty of Sciences, University of Malaga, E-29071, Malaga, Spain.
Abstract:
A method for the simultaneous spectrophotometric determination of the divalent ions of iron, cobalt, nickel and copper based on the formation of their complexes with 1,5-bis(di-2-pyridylmethylene) thiocarbonohydrazide (DPTH) is proposed. The resolution of quaternary mixtures of these metallic ions was accomplished by several chemometric approaches. A comparative study of the results obtained for simultaneous determinations in mixture by using principal component regression (PCR) and partial least-squares regression (PLS-1 and PLS-2) for absorbance, first-derivative and second-derivative data is presented. In general, the best recovery values are obtained by the PLS-2 method for absorbance data. This procedure allows the simultaneous determination of the cited ions in alloys and biological materials Good reliability of the determination was proved.
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