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Updated: Jun 28, 2026

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Analysis of ephedrine in ephedra callus by acetonitrile modified capillary zone electrophoresis
1Department of Chemistry, Lanzhou University, Lanzhou, People's Republic of China.
Abstract:
A simple method has been developed for the quantitative determination of ephedrine in ephedra callus. The dependence of effective mobility of ephedrine on pH was investigated, and a simulated equation was obtained. The separation was performed in an uncoated capillary and detected at 185 nm. A new Tris-NaOH-H(3)PO(4) run buffer was used and the pH was adjusted to 3.20. To increase the solubility of hydrophobic analytes and improve the separation efficiency, 15% acetonitrile was used in the buffer as a modifier. The content of ephedrine in an ephedra callus sample and an ephedra herba sample were determined with this method, and the result was satisfactory.
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